CodexBiosolutions provides cell based assay products and services to academic and governmental research institutes, pharmaceutical and biotechnology industry. The company is headquartered in the I-270 Hi-Tech corridor in the state of Maryland. The core products of CodexBiosolution are ACTOne Cell lines and membrane potential kits where are licensed from BD Biosciences. We also develop other new cell based products to meet the demands from various customers.
ACTOne Assay Principle
ACTOne Technology is the first in its class in providing real-time information on intracellular cAMP changes in a high-throughput format without a cell lysis step. The assay works with cell lines that contain a proprietary exogenous Cyclic Nucleotide-Gated (CNG) channel. The channel is activated by elevated intracellular levels of cAMP, resulting in ion flux (often detectable by calcium-responsive dyes) and cell membrane depolarization which can be detected with a fluorescent membrane potential (MP) dye. The ACTOne cAMP assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader. It is a simple homogenous assay involving only dye and compound addition steps, allowing easy implementation in a high-throughput environment. It has been successfully used to measure Gs and Gi coupled GPCR activity.